An Easily Made Tissue Culture Perfusion Chamber

A perfusion chamber is described with which a tissue culture can be irrigated successively with different immersion media. Only 1-2 ml. of medium are required for each change, which can be accomplished within 1 minute. Simplicity of design permits speed and economy in manufacture. Perfusion by suction instead of by positive pressure has eliminated certain drawbacks of previous designs. Mixing effects in the chamber have been studied. Any component of an immersion medium can be reduced to 0-5% of its initial concentration by the use of only 2 c.c. of fresh medium. The chamber has been used in the techniques of immersion refractometry and interferometry of living cells.